6533b851fe1ef96bd12a90a5

RESEARCH PRODUCT

Screening for multiple hereditary hypercoagulability factors using the amplification refractory mutation system

G. A. HeymannAbdulgabar SalamaBerthold HoppePeter HellsternHolger KiesewetterJürgen Koscielny

subject

GeneticsBase SequenceGenetic Carrier ScreeningHomozygoteGenetic Carrier ScreeningSingle-strand conformation polymorphismBlood ProteinsHematologyBlood Coagulation DisordersBiologymedicine.diseaseThrombophiliaBioinformaticsPolymerase Chain ReactionThrombosisBlood Coagulation FactorsRefractoryMutation (genetic algorithm)medicineCoagulopathyHumansMass ScreeningRisk factorDNA Primers

description

Many hereditary factors have been implicated in the development of arterial and/or venous thromboembolic diseases. A number of these risk factors can be identified by the amplification refractory mutation system (ARMS). However, the underlying technical conditions for performing ARMS are highly variable, and depend on which risk factors are being analyzed. We have now developed a novel ARMS-based system to simultaneously screen for multiple hypercoagulability factors under identical PCR conditions. This can greatly simplify the process of screening for hereditary hypercoagulability.

yearjournalcountryeditionlanguage
2003-12-04Thrombosis Research
https://doi.org/10.1016/j.thromres.2003.08.024