6533b851fe1ef96bd12aa1eb

RESEARCH PRODUCT

Anti-phospholipase A2 and anti-inflammatory activity of Santolina chamaecyparissus

José-luis RíosAraceli SalaRosa M. GinerM. Carmen RecioSalvador Máñez

subject

medicine.drug_classAsteraceaePharmacologyFlavonesPhospholipases AGeneral Biochemistry Genetics and Molecular BiologyAnti-inflammatorySantolina chamaecyparissusMicechemistry.chemical_compoundPhospholipase A2medicineAnimalsEnzyme InhibitorsGeneral Pharmacology Toxicology and PharmaceuticsFlavonoidschemistry.chemical_classificationPlants MedicinalbiologyPlant ExtractsChemistryAnti-Inflammatory Agents Non-SteroidalGeneral Medicinebiology.organism_classificationIn vitroPhospholipases A2BiochemistrySephadexbiology.proteinFemalelipids (amino acids peptides and proteins)Arachidonic acidSesquiterpenesNepetin

description

The activity of the Santolina chamaecyparissus methanol extract was tested against the phospholipase A2 (PLA2)-induced mouse paw edema and in vitro inhibition of PLA2 activity. After fractionation, only the dichloromethane extract was active against the PLA2 in vitro test. In addition, it reduced the edema induced by arachidonic acid, and by 12-O-tetradecanoylphorbol-13-acetate in a multidose test. After chromatography on silicagel and gel filtration on Sephadex, and using an in vitro anti-PLA2 assay-guided process, we have isolated and identified from the dichloromethane extract the flavone nepetin and four sesquiterpenes.

yearjournalcountryeditionlanguage
2000-02-09Life Sciences
https://doi.org/10.1016/s0024-3205(99)00578-0