HPLC Method for Cyst(e)ine and Methionine in Infant Formulas

6533b851fe1ef96bd12aa1f0

RESEARCH PRODUCT

HPLC Method for Cyst(e)ine and Methionine in Infant Formulas

Reyes Barberá María Jesús Lagarda J.c. López A. Alegría Toran Rosaura Farré

subject

chemistry.chemical_compound Performic acid Chromatography Methionine Column chromatography chemistry Acid hydrolysis Cysteic acid Derivatization High-performance liquid chromatography Food Science Cysteine

description

Cyst(e)ine and methionine were converted into cysteic acid and methionine sulfone by oxidizing with performic acid. The oxidized samples were then subjected to acid hydrolysis (6N HCl, 105-110°C/24 hr). After derivatization with phenylisothiocyanate, reverse phase HPLC separation was carried out at 48°C and with UV detection. Different gradients and pH values in the eluent were assayed to determine the best resolution. Analytical parameters, detection and quantification limits, linearity, precision and accuracy, were determined. The method was reliable and accurate for measuring cyst(e)ine and methionine in infant formulae.

year	journal	country	edition	language
1996-11-01	Journal of Food Science

https://doi.org/10.1111/j.1365-2621.1996.tb10946.x

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