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RESEARCH PRODUCT
EFFECTS OF VANADATE ON RESPONSES OF GUINEA-PIG ISOLATED TRACHEA TO SPASMOGENS
V. VillagrasaM AntónJulio CortijoEsteban J. MorcilloPedro Santamarı́asubject
Malemedicine.medical_specialtySerotoninContraction (grammar)Guinea PigsPharmaceutical ScienceIn Vitro TechniquesPotassium ChlorideContractilitychemistry.chemical_compoundInternal medicineCaffeinemedicineAnimalsVanadatePharmacologyChemistryCell MembraneMuscle SmoothAcetylcholineTracheaEGTAEndocrinologyParasympathomimeticsVerapamilVerapamilCalciumFemalemedicine.symptomVanadatesAcetylcholineHistaminemedicine.drugMuscle contractionHistamineMuscle Contractiondescription
Abstract The effects of vanadate on the contractility of the guinea-pig isolated trachea was examined. Vanadate (0·1 Mm) produced a sustained contraction that was abolished in Ca2+-free EGTA (0·1 Mm)-containing physiological salt solution but was resistant to verapamil (1 μm). Vanadate (0·1 Mm) depressed tracheal responses to CaCl2 (in Ca2+-free depolarizing solution), KCl, acetylcholine, histamine and 5-hydroxytryptamine. For vanadate (10 μm), the inhibition of spasmogenic responses only reached statistical significance for histamine and 5-hydroxytryptamine. Caffeine (1 Mm)-induced spasm (trachea at 20°C in the presence of indomethacin (2·8 μm)) was not affected by vanadate (10 μm-0·1 Mm). Vanadate (0·1 Mm) slightly depressed the responses to KCl (50 Mm), acetylcholine (1 Mm), histamine (1 Mm) or 5-hydroxytryptamine (0·1 Mm) observed in Ca2+-free EGTA (0·1 Mm)-containing physiological salt solution. Vanadate (0·5 Mm) depressed Ca2+ (20 μm)-induced contraction of trachea which had been chemically skinned of its plasmalemmal membranes. The mechanism of the inhibitory effect of vanadate on tracheal responses to a variety of spasmogens remains obscure, but, under in-vitro conditions, vanadate clearly does not induce hyper-reactivity of airway smooth muscle to spasmogens.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-01-01 |