Bile acid receptor TGR5 is critically involved in preference for dietary lipids and obesity

6533b852fe1ef96bd12ab7b1

RESEARCH PRODUCT

Bile acid receptor TGR5 is critically involved in preference for dietary lipids and obesity

Hayet Oulamara A.-n. Agli Amira Sayed Khan Aziz Hichami Mustapha Berrichi Adel Bensalem Babar Murtaza Gregory Merlen Thierry Tordjmann Naim Akhtar Khan

subject

Blood Glucose Lipopolysaccharides Male 0301 basic medicine [SDV]Life Sciences [q-bio]Endocrinology Diabetes and Metabolism Clinical Biochemistry Biochemistry Receptors G-Protein-Coupled Mice 0302 clinical medicine Insulin Receptor Mice Knockout 2. Zero hunger chemistry.chemical_classification Nutrition and Dietetics Lipids G protein-coupled bile acid receptor [SDV] Life Sciences [q-bio]medicine.anatomical_structure medicine.medical_specialty Mice Transgenic 030209 endocrinology & metabolism Single-nucleotide polymorphism Diet High-Fat Polymorphism Single Nucleotide Bile Acids and Salts Food Preferences 03 medical and health sciences Internal medicine Taste bud medicine Animals Obesity Molecular Biology Inflammation business.industry Taste bud Fatty acid Fatty acid medicine.disease Dietary Fats Obesity In vitro Fatty Liver Mice Inbred C57BL Disease Models Animal 030104 developmental biology Endocrinology chemistry Fat Calcium Steatosis business

description

International audience; We investigated the implication of Takeda G protein-coupled receptor 5 (TGR5) in fat preference and fat sensing in taste bud cells (TBC) in C57BL/6 wild-type (WT) and TGR5 knock out (TGR5-/-) male mice maintained for 20 weeks on a high-fat diet (HFD). We also assessed the implication of TGR5 single nucleotide polymorphism (SNP) in young obese humans. The high-fat diet (HFD)-fed TGR5-/- mice were more obese, marked with higher liver weight, lipidemia and steatosis than WT obese mice. The TGR5-/- obese mice exhibited high daily food/energy intake, fat mass and inflammatory status. WT obese mice lost the preference for dietary fat, but the TGR5-/- obese mice exhibited no loss towards the attraction for lipids. In lingual TBC, the fatty acid-triggered Ca2+ signaling was decreased in WT obese mice; however, it was increased in TBC from TGR5-/- obese mice. Fatty acid-induced in vitro release of GLP-1 was higher, but PYY concentrations were lower, in TBC from TGR5-/- obese mice than those in WT obese mice. We noticed an association between obesity and variations in TGR5 rs11554825 SNP. Finally, we can state that TGR5 modulates fat eating behavior and obesity.

year	journal	country	edition	language
2020-02-29	The Journal of Nutritional Biochemistry

https://doi.org/10.1016/j.jnutbio.2019.108298