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RESEARCH PRODUCT
Comparison of an indirect format ELISA on modified graphite and polystyrene surfaces against triazines.
Iris SoundSaid A. IbrahimA. M. HammamDina M. FouadWolfram BaumannUrsula Pfeifer-fukumuraHeike Holthuessubject
chemistry.chemical_classificationAnalyteChromatographySurface PropertiesTriazinesEnzyme-Linked Immunosorbent AssayTerbuthylazineAldehydeGeneral Biochemistry Genetics and Molecular Biologychemistry.chemical_compoundchemistryPolystyrenesGraphiteGraphitePolystyreneGlutaraldehydeHaptenHaptensAlkyldescription
An indirect format enzyme-linked immuno-sorbent assay (ELISA) on graphite rods (Ø 0.8 mm x 20 mm) and, for comparison, on microtiter plates has been developed against terbuthylazine. For this purpose, a series of 2-aminoalkyl-4-chloro-6-terbuthyl-s-triazine-2,6- diamine ELISA haptens with alkyl spacer lengths of 2, 4, 6, and 8 CH2 groups has been synthesized. The graphite rods or the microtiter plates were covered by a polymerized glutaraldehyde network, and the ELISA haptens have been coupled by imino coupling to the free aldehyde groups of that network. ε-Aminocaproic acid has been used as an agent to block unspecific binding sites. The ELISA was run in a competitive mode, where the immobilized ELISA hapten and the solute terbuthylazine as a target analyte compete for the solute antibody.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-05-30 | Zeitschrift fur Naturforschung. C, Journal of biosciences |