6533b853fe1ef96bd12ac198
RESEARCH PRODUCT
“BEP” RNAs and Proteins Are Situated in the Animal Side of Sea Urchin Unfertilized Egg, Which Can Be Recognized by Female Pronuclear Localization
Giuseppina OrtolaniMarta Di CarloDaniele P. RomancinoGiovanni GiudiceGiovanna Montanasubject
BiophysicsIn situ hybridizationBiochemistryParacentrotus lividusbiology.animalBotanyMorphogenesismedicineAnimalsRNA MessengerMolecular BiologySea urchinOvumCell NucleusbiologyPronucleusMembrane ProteinsRNACell Biologybiology.organism_classificationCell biologyCell nucleusmedicine.anatomical_structureMembrane proteinSea Urchinsembryonic structuresRNAFemaleImmunostainingdescription
Microsurgery experiments demonstrate that the animal side of the unfertilized sea urchin Paracentrotus lividus egg coincides with the side of the egg pronucleus location. It is demonstrated by means of in situ hybridization and immunostaining of whole mounts of animal or vegetal halves that the previously identified bep 1 and bep4 RNAs and their proteins are located in the animal part of the unfertilized egg and much less in the vegetal part. The addition of Fabs against BEP1 and BEP4 causes exogastrulation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-12-13 | Biochemical and Biophysical Research Communications |