6533b853fe1ef96bd12ad79e
RESEARCH PRODUCT
Protective Effect of Cactus Cladode Extracts on Peroxisomal Functions in Microglial BV-2 Cells Activated by Different Lipopolysaccharides
Norbert LatruffeFatima-ezzahra SaihBoubker NasserM'hammed Saïd El KebbajStéphane MandardPierre AndreolettiGérard LizardMustapha Cherkaoui-malkisubject
0301 basic medicineAntioxidant[SDV]Life Sciences [q-bio]medicine.medical_treatmentAnti-Inflammatory AgentsPharmaceutical Scienceacyl-CoA oxidase 1; catalase; β-oxidation; <i>Escherichia coli</i>; lipopolysaccharides; LPS; nitric oxide; Opuntia; peroxisomes; <i>Salmonella minnesota</i>AntioxidantsAnalytical ChemistryMicechemistry.chemical_compoundSalmonellaDrug Discoverychemistry.chemical_classificationbiologyMicrogliaFatty AcidscatalaseOpuntiaPeroxisome[SDV] Life Sciences [q-bio]Neuroprotective Agentsmedicine.anatomical_structureBiochemistryChemistry (miscellaneous)CatalaseMolecular MedicineACOX1Microgliamedicine.symptomOxidation-ReductionLPSInflammationArticleCell LineNitric oxideMicrobiologylcsh:QD241-44103 medical and health scienceslcsh:Organic chemistrynitric oxideEscherichia colimedicineAnimalsSalmonella minnesotaPhysical and Theoretical Chemistryacyl-CoA oxidase 1[ SDV ] Life Sciences [q-bio]Plant ExtractsOrganic ChemistryperoxisomeslipopolysaccharidesOxidative Stress030104 developmental biologyEnzymechemistrybiology.proteinβ-oxidationReactive Oxygen Speciesdescription
International audience; In this study, we aimed to evaluate the antioxidant and anti-inflammatory properties of Opuntia ficus-indica cactus cladode extracts in microglia BV-2 cells. Inflammation associated with microglia activation in neuronal injury can be achieved by LPS exposure. Using four different structurally and biologically well-characterized LPS serotypes, we revealed a structure-related differential effect of LPS on fatty acid β-oxidation and antioxidant enzymes in peroxisomes: Escherichia coli-LPS decreased ACOX1 activity while Salmonella minnesota-LPS reduced only catalase activity. Different cactus cladode extracts showed an antioxidant effect through microglial catalase activity activation and an anti-inflammatory effect by reducing nitric oxide (NO) LPS-dependent production. These results suggest that cactus extracts may possess a neuroprotective activity through the induction of peroxisomal antioxidant activity and the inhibition of NO production by activated microglial cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-01-07 | Molecules |