6533b854fe1ef96bd12ae937

RESEARCH PRODUCT

Inflammation-induced upregulation of NK1 receptor mRNA in dorsal horn neurones

Eberhard WeiheJames E. KrauseMartin K.-h. SchäferD. Nohr

subject

Malemedicine.medical_specialtyCentral nervous systemSubstance PIn situ hybridizationNeurotransmissionSubstance-P Receptorchemistry.chemical_compoundInternal medicineImage Processing Computer-AssistedmedicineAnimalsRNA MessengerRats WistarReceptorIn Situ HybridizationInflammationNeuronsChemistryGeneral NeuroscienceAnatomyReceptors Neurokinin-1Spinal cordHindlimbRatsUp-Regulationmedicine.anatomical_structureNociceptionEndocrinologySpinal Cordnervous systemAutoradiography

description

The expression of the neurokinin 1 (NK1) receptor (i.e. substance P receptor) gene in spinal cord was studied in rats subjected to unilateral inflammation by semi-quantitative in situ hybridization analysis. Low levels of NK1 receptor mRNA were detected in many neurones throughout the grey matter. Relatively strong labelling was observed in large motoneurones and a subpopulation of superficial dorsal horn neurones. Six days after Freund's adjuvant-induced unilateral hindpaw inflammation, NK1 receptor mRNA levels in lamina I/II of the dorsal horn ipsilateral to the inflamed paw increased almost two fold compared with the contralateral side. These data suggest an inflammation-induced increase of NK1 receptor synthesis in intrinsic spinal cord neurones involved in nociceptive neurotransmission.

yearjournalcountryeditionlanguage
1993-08-01NeuroReport
https://doi.org/10.1097/00001756-199308000-00003