An in vitro model for the study of human parathyroid gland tissue: Single cell suspensions and monolayer cultures

6533b855fe1ef96bd12b120c

RESEARCH PRODUCT

An in vitro model for the study of human parathyroid gland tissue: Single cell suspensions and monolayer cultures

H. Gabbert M. Rothmund Th. Schärfe P. K. Wagner U. Krause A. Knuth

subject

Adenoma Cell chemistry.chemical_element Parathyroid hormone Calcium Models Biological Cryopreservation law.invention Parathyroid Glands law medicine Humans Magnesium Secretion Cells Cultured chemistry.chemical_classification Magnesium General Medicine Parathyroid Neoplasms medicine.anatomical_structure Enzyme chemistry Biochemistry Parathyroid Hormone Calcium Female Electron microscope hormones hormone substitutes and hormone antagonists

description

An in vitro model for studies of parathyroid physiology is described using single cell suspensions and adherent monolayer cultures of human parathyroid tissue. The isolated cells were viable and maintained functional properties tested by calcium and magnesium sensitivity. Parathyroid hormone (PTH) secretion could be suppressed by rising calcium and magnesium concentrations. The secretory behaviour of the cells was not altered by cultivation or cryopreservation. The morphological integrity of the individual cells after mechanical and enzymatic preparation was confirmed by light and electron microscopy.

year	journal	country	edition	language
1982-06-01	Research in Experimental Medicine

https://doi.org/10.1007/bf01852191

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