6533b856fe1ef96bd12b246f

RESEARCH PRODUCT

Tunable axial superresolution by annular binary filters. Application to confocal microscopy

Jorge Ojeda-castanedaManuel Martínez-corralGenaro SaavedraPedro Andrés

subject

Point spread functionMaterials sciencebusiness.industryBinary numberSuperresolutionAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic Materialslaw.inventionReduction (complexity)OpticsApodizationConfocal microscopylawCentral lobeElectrical and Electronic EngineeringPhysical and Theoretical Chemistrybusiness

description

We present a set of annular binary pupil filters for increasing the axial resolving capacity of imaging systems. The filters consist of two transparent annuli of the same area. It is shown that by changing the area of the transparent regions it is possible to obtain a tunable reduction of the width of the central lobe of the axial point spread function of the imaging system. However, this reduction is accompanied by a severe increase of the strength of secondary lobes, what can make these filters not very useful when used in conventional imaging systems. That is why we propose to use these filters for apodizing confocal microscopy systems. It is shown that in this case an important reduction is achieved in the volume of the central lobe of the three-dimensional point spread function.

https://doi.org/10.1016/0030-4018(95)00380-q