6533b856fe1ef96bd12b28b2
RESEARCH PRODUCT
Caffeine increases the expression of cystatin SN in human submandibular acinar-like HSG cells
Martine MorzelMartine MorzelMartine MorzelIsabelle SeverinIsabelle SeverinIsabelle SeverinLudovic Le CorreMicheline DsamouMicheline DsamouMicheline DsamouMarie-christine ChagnonMarie-christine ChagnonMarie-christine Chagnonsubject
medicine.medical_specialtySalivaCellular differentiationeducationCell Culture TechniquesEnzyme-Linked Immunosorbent Assay[SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain03 medical and health scienceschemistry.chemical_compound0302 clinical medicinestomatognathic systemInternal medicineCaffeinemedicineHumansViability assaySalivaGeneral DentistryBitterness030304 developmental biology0303 health sciencesMatrigelSubmandibular glandChemistryCell BiologyGeneral MedicineSubmandibular glandIn vitroDrug CombinationsEndocrinologymedicine.anatomical_structureOtorhinolaryngologyCell culture030220 oncology & carcinogenesisSalivary CystatinsProteoglycansHSG cell lineCollagenLamininCaffeinedescription
The study aimed at evaluating in vitro the effect of caffeine on expression of cystatin SN, a potential marker of sensitivity to bitterness in humans.Differentiation of human submandibular gland (HSG) cells was induced by culturing cells on Matrigel. Caffeine cytotoxicity was assessed over 3 days by the Resazurin test. Finally, effects of 5, 50 and 100μM caffeine exposure on cystatin SN expression were explored over 3 days by ELISA.At concentrations relevant to human adult plasma levels (5, 50 and 100μM), caffeine did not affect cell viability whether cells were differentiated or not. Cystatin SN levels were overall higher in differentiated cells and increased with time in both conditions. There was a significant (p0.001) effect of caffeine on cystatin SN expression specifically in differentiated cells.The HSG cell line proved to be a relevant tool to study in vitro the effect of caffeine at concentrations consistent with dietary intake in human subjects. The results suggest that salivary cystatin SN abundance may depend on caffeine intake, with possible consequences on taste sensitivity.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-10-01 |