6533b856fe1ef96bd12b2e8e

RESEARCH PRODUCT

Indirect determination of paracetamol in pharmaceutical formulations by inhibition of the system luminol–H2O2–Fe(CN)63− chemiluminescence

A. Gregorio AlapontL. Lahuerta ZamoraJ. Martínez Calatayud

subject

ChromatographyCalibration curveClinical BiochemistryAnalgesicAnalytical chemistryPharmaceutical ScienceDosage formAnalytical ChemistryLuminollaw.inventionchemistry.chemical_compoundchemistrylawDrug DiscoveryFerricyanideHydrogen peroxideQuantitative analysis (chemistry)SpectroscopyChemiluminescence

description

After a large drug scanning, the system Luminol-H2O2-Fe(CN)6(3-) is proposed for first time for the indirect determination of paracetamol. The method is based on the oxidation of paracetamol by hexacyanoferrate (III) and the subsequent inhibitory effect on the reaction between luminol and hydrogen peroxide. The procedure resulted in a linear calibration graph over the range 2.5-12.5 microg ml(-1) of paracetamol with a sample throughput of 87 samples h(-1). The influence of foreign compounds was studied and, the method was applied to determination of the drug in three different pharmaceutical formulations.

yearjournalcountryeditionlanguage
1999-11-01Journal of Pharmaceutical and Biomedical Analysis
https://doi.org/10.1016/s0731-7085(99)00124-7