6533b856fe1ef96bd12b2fc8
RESEARCH PRODUCT
Single Molecule Spectroscopy of Oriented Recombinant Trimeric Light Harvesting Complexes of Higher Plants
Henriette Wolf-kleinFedor JelezkoJörg WrachtrupUwe GerkenBritta GötzeChristine HuschenbettS. SchulerC. TietzHarald Paulsensubject
Aqueous solutionProtein subunitClinical BiochemistryGeneral ChemistryPhotochemistryPhotobleachingGeneral Biochemistry Genetics and Molecular BiologySingle Molecule Spectroscopylaw.inventionLight-harvesting complexCoupling (electronics)chemistry.chemical_compoundCrystallographyMonomerchemistrylawRecombinant DNAMolecular Biologydescription
The bleaching dynamics of reconstituted single light-harvesting chlorophyll a/b investigated. The complexes containing one histidine6 tag per monomeric subunit were immobilised predominantly in a defined orientation with their symmetry axis perpendicular to a Ni-ion-containing surface allowing for the first time the examination of single LHCIIb in an aqueous environment. Most complexes exhibit photobleaching in one step, indicating coupling between the monomeric subunits leading to an energy transfer between adjacent subunits. Differences in bleaching behaviour between these and previous observations with single LHCIIb are discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-08-01 | Single Molecules |