6533b857fe1ef96bd12b4e4e
RESEARCH PRODUCT
In-situ gelling xyloglucan formulations as 3D artificial niche for adipose stem cell spheroids.
Clelia DispenzaA. Di StefanoFrancesca ToiaE. MuscolinoDaniela GiacomazzaFrancesco MoschellaMaria Antonietta SabatinoAdriana Cordovasubject
Cell SurvivalPopulationCellCell Culture TechniquesAdipose tissue02 engineering and technology[object Object]Biochemistry03 medical and health scienceschemistry.chemical_compoundStructural BiologySpheroids CellularmedicineHumansViability assayeducationMolecular BiologyGlucansCells Cultured030304 developmental biology0303 health scienceseducation.field_of_studyMicroscopyTissue EngineeringViscosityRegeneration (biology)SOXB1 Transcription FactorsSpheroids of adipose stem cells Artificial niche In-situ forming gel Partially degalactosylated xyloglucanSpheroidHydrogelsMesenchymal Stem CellsGeneral MedicineNanog Homeobox Protein021001 nanoscience & nanotechnologyCell biologyCulture MediaXyloglucanmedicine.anatomical_structurechemistryMicroscopy Electron ScanningXylansSettore CHIM/07 - Fondamenti Chimici Delle TecnologieStem cell0210 nano-technologyRheologyShear StrengthOctamer Transcription Factor-3description
Abstract Three-dimensional spheroidal cell aggregates of adipose stem cells (SASCs) are a distinct upstream population of stem cells present in adipose tissue, with enhanced regeneration properties in vivo. The preservation of the 3D structure of the cells, from extraction to administration, can be a promising strategy to ensure optimal conditions for cell viability and maintenance of stemness potential. With this aim, an artificial niche was created by incorporating the spheroids into an injectable, in-situ gelling solution of partially degalactosylated xyloglucan (dXG) and an ad hoc formulated culture medium for the preservation of stem cell spheroid features. The evolution of the mechanical properties and the morphological structure of this artificial niche was investigated by small amplitude rheological analysis and scanning electron microscopy, respectively. Comparatively, systems produced with the same polymer and the typical culture medium (DMEM) used for adipose stem cell (ASC) growth in adherent cell culture conditions were also characterised. Cell viability of both SASCs and ASCs incorporated inside the hydrogel or seeded on top of the hydrogel were investigated as well as the preservation of SASC stemness conditions when embedded in the hydrogel.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-01-01 | International journal of biological macromolecules |