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RESEARCH PRODUCT
Human Spheroids from Adipose-Derived Stem Cells Induce Calvarial Bone Production in a Xenogeneic Rabbit Model
Francesco MoschellaCesare GagliardoAngelo A. Leto BaroneAda Maria FlorenaGiuseppa BilelloAnna Barbara Di StefanoFrancesca ToiaLuigi MontesanoAdriana CordovaAlessandro GulinoBeatrice Belmontesubject
Bone RegenerationCellular differentiationAdipose tissueBone healing030230 surgerySettore MED/08 - Anatomia Patologica03 medical and health sciences0302 clinical medicineTissue engineeringOsteogenesisAdipocytesAnimalsHumansMedicinespheroids.Bone regenerationCells Culturedadipose-derived stem cellbusiness.industryOssificationStem CellsRegeneration (biology)SkullCell DifferentiationCell biologyAdipose Tissue030220 oncology & carcinogenesisSurgeryRabbitsmedicine.symptomStem cellbusinessdescription
ABSTRACT: Calvarial defects can result from several causes. Tissue engineering hold the potential to restore native form and protective function. We have recently shown that stemness and differentiation ability of spheroids from adipose-derived stem cells (S-ASCs) promotes osteoblasts growth within Integra in a small vertebral lesion. In our study, we aimed to test osteogenic potential of S-ASCs in aiding regeneration of a calvarial defect. Groups containing Integra showed increased bone regeneration at the calvarial defect-Integra interface compared with the control group. In particular, S-ASC-derived osteoblasts group showed a superior calvarial remodeling than undifferentiated S-ASCs group. Clusters of ossification were observed in these both groups with enhanced microvasculature density and fibrosis. In conclusion, seeding of S-ASCs in dermal regeneration templates enhanced bone healing in a rabbit calvarial defect model. These findings could prompt the elective use of S-ASCs with enhanced multilineage differentiation potential for tissue engineering purposes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-12-17 |