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RESEARCH PRODUCT
Optical-sectioning improvement in two-color excitation scanning microscopy
Isabel EscobarManuel Martínez-corralC. Ibáñez-lópezGenaro Saavedrasubject
HistologyMaterials scienceMicroscopeOptical sectioningbusiness.industrylaw.inventionMedical Laboratory TechnologyWavelengthOpticsTwo-photon excitation microscopylawLight sheet fluorescence microscopyMicroscopyFluorescence microscopeAnatomybusinessInstrumentationExcitationdescription
We present a new beam-shaping technique for two-color excitation fluorescence microscopy. We show that by simply inserting a properly designed shaded-ring filter in the illumination beam of smaller wavelength, it is possible to improve the effective optical sectioning capacity of such microscopes by 23%. Such an improvement is obtained at the expense of only a very small increasing of the overall energy in the point-spread-function sidelobes. The performance of this technique is illustrated by a numerical imaging simulation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-06-01 | Microscopy Research and Technique |