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RESEARCH PRODUCT

Analysis of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage with High Frequency Transcranial Duplex Ultrasound

Michael KosterhonPhilip WenzelSerge C. ThalSusanne KarbachFlorian RingelAxel NeulenMichael MolitorTobias PantelThomas Gaul

subject

medicine.medical_specialtySubarachnoid hemorrhageUltrasonography Doppler TranscranialGeneral Chemical EngineeringIschemiaGeneral Biochemistry Genetics and Molecular BiologyBrain IschemiaMiceCerebral vasospasmIn vivoInternal medicinemedicineAnimalsVasospasm Intracranialcardiovascular diseasesStrokeGeneral Immunology and Microbiologybusiness.industryGeneral NeuroscienceUltrasoundVasospasmBlood flowSubarachnoid Hemorrhagemedicine.diseasenervous system diseasesDisease Models Animalcardiovascular systemCardiologybusinessBlood Flow Velocity

description

Cerebral vasospasm that occurs in the weeks after subarachnoid hemorrhage, a type of hemorrhagic stroke, contributes to delayed cerebral ischemia. A problem encountered in experimental studies using murine models of SAH is that methods for in vivo monitoring of cerebral vasospasm in mice are lacking. Here, we demonstrate the application of high frequency ultrasound to perform transcranial Duplex sonography examinations on mice. Using the method, the internal carotid arteries (ICA) could be identified. The blood flow velocities in the intracranial ICAs were accelerated significantly after induction of SAH, while blood flow velocities in the extracranial ICAs remained low, indicating cerebral vasospasm. In conclusion, the method demonstrated here allows functional, noninvasive in vivo monitoring of cerebral vasospasm in a murine SAH model.

yearjournalcountryeditionlanguage
2021-06-03Journal of Visualized Experiments
https://doi.org/10.3791/62186