6533b858fe1ef96bd12b6346

RESEARCH PRODUCT

Rapid hyperpolarization and purification of the metabolite fumarate in aqueous solution

John W. BlanchardLaurynas DagysDmitry BudkerEleonora CavallariMalcolm H. LevittGerd BuntkowskyMaksim TsukanovDanila A. BarskiyAlexander PinesFrancesca ReineriKerstin MünnemannStephan KnechtSilvio AimeJames EillsErik Van DykePeter BlümlerBea Bliemel

subject

Molar concentrationparahydrogen02 engineering and technologyBiosensing Techniques010402 general chemistry01 natural sciencesChemical reaction41003 medical and health sciences0302 clinical medicineFumaratesHyperpolarization (physics)Carbon-13 Magnetic Resonance SpectroscopyPolarization (electrochemistry)DissolutionhyperpolarizationBiomarker; Hyperpolarization; Metabolism; MRI; Parahydrogen; Fumarates; Molecular Imaging; Solutions; Water; Biosensing Techniques; Carbon-13 Magnetic Resonance Spectroscopychemistry.chemical_classificationParahydrogenMultidisciplinaryAqueous solutionChemistryBiomolecule500WaterBiomarker021001 nanoscience & nanotechnologyCombinatorial chemistryMolecular Imaging0104 chemical sciencesSolutionsSolventChemistryHyperpolarizationMetabolism030220 oncology & carcinogenesisReagentPhysical Sciencesbiomarkerddc:5000210 nano-technologymetabolismBiosensorMRI

description

Significance Magnetic resonance imaging is hindered by inherently low sensitivity, which limits the method for the most part to observing water molecules in the body. Hyperpolarized molecules exhibit strongly enhanced MRI signals which opens the door for imaging low-concentration species in vivo. Biomolecules can be hyperpolarized and injected into a patient allowing for metabolism to be tracked in real time, greatly expanding the information available to the radiologist. Parahydrogen-induced polarization (PHIP) is a hyperpolarization method renowned for its low cost and accessibility, but is generally limited by low polarization levels, modest molecular concentrations, and contamination by polarization reagents. In this work we overcome these drawbacks in the production of PHIP-polarized [1-13C]fumarate, a biomarker of cell necrosis in metabolic 13C MRI.

yearjournalcountryeditionlanguage
2020-09-03
https://dx.doi.org/10.26083/tuprints-00024221