6533b859fe1ef96bd12b6ea1
RESEARCH PRODUCT
Correlative Raman-Electron-Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells.
Francesca PagliariElisa SogneDavide PanellaGerardo PerozzielloCarlo LiberaleGobind DasAlice TurdoSimone Di FrancoJoao SecoAndrea FalquiSanto GratteriArturo PujiaEnzo Di FabrizioPatrizio CandeloroLuca Tirinatosubject
electron microscopyClinical BiochemistrySettore MED/50 - Scienze Tecniche Mediche ApplicateBiomedical Engineeringmelanoma cancer stem cellRaman micro-imaginglipid dropletElectronsGeneral MedicineLipid DropletsSpectrum Analysis RamanAnalytical Chemistrycorrelative microscopy; lipid droplets; melanoma cancer stem cells; Raman micro-imaging; electron microscopyMicroscopy Electron ScanningNeoplastic Stem CellsHumanscorrelative microscopySettore MED/46 - Scienze Tecniche Di Medicina Di LaboratorioInstrumentationEngineering (miscellaneous)MelanomaBiotechnologydescription
Among all neoplasms, melanoma is characterized by a very high percentage of cancer stem cells (CSCs). Several markers have been proposed for their identification, and lipid droplets (LDs) are among them. Different techniques are used for their characterization such as mass spectrometry, imaging techniques, and vibrational spectroscopies. Some emerging experimental approaches for the study of LDs are represented by correlative light–electron microscopy and by correlative Raman imaging–scanning electron microscopy (SEM). Based on these scientific approaches, we developed a novel methodology (CREL) by combining Raman micro-spectroscopy, confocal fluorescence microscopy, and SEM coupled with an energy-dispersive X-ray spectroscopy module. This procedure correlated cellular morphology, chemical properties, and spatial distribution from the same region of interest, and in this work, we presented the application of CREL for the analysis of LDs within patient-derived melanoma CSCs (MCSCs).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2022-12-01 | Biosensors |