6533b859fe1ef96bd12b777d
RESEARCH PRODUCT
Leucine aminopeptidase is an immunodominant antigen of Fasciola hepatica excretory and secretory products in human infections.
José-guillermo EstebanDolores BernalDaniel AcostaJosé F. TortJ. De La RubiaCarlos CarmonaZ. VillavicencioJavier SotilloF. J. BornayAntonio MarcillaRafael Toledosubject
Microbiology (medical)FascioliasisAdolescentClinical BiochemistryImmunologyBlotting WesternMolecular Sequence DataSheep DiseasesEnzyme-Linked Immunosorbent AssayAminopeptidasePolymerase Chain ReactionLeucyl AminopeptidaseImmune systemAntigenHepaticaparasitic diseasesmedicineImmunology and AllergyFasciola hepaticaAnimalsHumansElectrophoresis Gel Two-DimensionalFasciolosisChildDNA PrimersSheepbiologyBase SequenceImmunodominant EpitopesClinical and Diagnostic Laboratory ImmunologyLiver flukeFasciola hepaticabiology.organism_classificationmedicine.diseaseVirologyExcretory systemAntigens HelminthChild Preschooldescription
ABSTRACT The liver fluke Fasciola hepatica parasitizes humans and ruminant livestock worldwide, and it is now being considered a reemerging zoonotic disease, especially in areas in which it is endemic, such as South America. This study investigates the immune response to excretory and secretory products produced by F. hepatica in a group of patients from the Peruvian Altiplano, where the disease is highly endemic. Using a proteomic approach and immunoblotting techniques, we have identified the enzymes leucine aminopeptidase (LAP) and phosphoenolpyruvate carboxykinase as immunodominant antigens recognized by sera from fasciolosis patients. An indirect enzyme-linked immunosorbent assay using recombinant LAP as the antigen was developed to check sera from individuals of this region. Our results demonstrate that LAP produces a specific and strong reaction, suggesting its potential use in the serologic diagnosis of F. hepatica infections in humans.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2007-11-16 | Clinical and vaccine immunology : CVI |