6533b859fe1ef96bd12b80d5
RESEARCH PRODUCT
Cell-cell recognition system in gorgonians: description of the basic mechanism
W. E. G. MüllerJürgen ConradRudolf K. ZahnG. UhlenbruckZ. KljajićIsabel M. MüllerHeinz-christoph Schrödersubject
EcologybiologyPetri dishCellCell-cell recognitionLectinAquatic ScienceTrypsinDissociation (chemistry)law.inventionmedicine.anatomical_structureBiochemistryCell–cell interactionlawbiology.proteinmedicineEcology Evolution Behavior and SystematicsIntracellularmedicine.drugdescription
The dissociation of the gorgonian Eunicella cavolinii (Koch) into single cells was successfully accomplished. These cells readily formed aggregates of a size of 2 100 μm during incubation in roller tubes; no aggregate formation was observed in non-rotating Petri dishes. The formation of aggregates was not influenced by Ca++, urea or trypsin; it was also independent of temperature (4° to 30°C) and pH (5.5–9.0). The intercellular material of the gorgonian contains a galactose-specific lectin, as determined by double diffusion experiments and haemagglutination inhibition experiments using a series of galactoglycoconjugates. This lectin converted the aggregation-susceptible cells to aggregation-deficient cells. These findings are summarized in a novel model, in which it is assumed that the lectin masks the function of the cell surface-associated aggregation molecules.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1983-09-01 | Marine Biology |