6533b859fe1ef96bd12b813c
RESEARCH PRODUCT
Three-dimensional real-time darkfield imaging through Fourier lightfield microscopy
Gabriele ScrofaniGenaro SaavedraManuel Martínez-corralEmilio Sánchez-ortigasubject
Materials scienceMicroscope02 engineering and technology01 natural sciencesLight scatteringlaw.invention010309 opticssymbols.namesakeOpticslaw0103 physical sciencesMicroscopybusiness.industryPerspective (graphical)Orthographic projectionReconstruction algorithmÒptica021001 nanoscience & nanotechnologySample (graphics)Atomic and Molecular Physics and OpticsMicroscòpiaFourier transformsymbols0210 nano-technologybusinessdescription
We report a protocol that takes advantage of the Fourier lightfield microscopy concept for providing 3D darkfield images of volumetric samples in a single-shot. This microscope takes advantage of the Fourier lightfield configuration, in which a lens array is placed at the Fourier plane of the microscope objective, providing a direct multiplexing of the spatio-angular information of the sample. Using the proper illumination beam, the system collects the light scattered by the sample while the background light is blocked out. This produces a set of orthographic perspective images with shifted spatial-frequency components that can be recombined to produce a 3D darkfield image. Applying the adequate reconstruction algorithm high-contrast darkfield optical sections are calculated in real time. The presented method is applied for fast volumetric reconstructions of unstained 3D samples.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-01-01 |