6533b859fe1ef96bd12b82d8

RESEARCH PRODUCT

ANO1 as a marker of oral squamous cell carcinoma and silencing ANO1 suppresses migration of human scc-25 cells

Yadong LiSuling HongJinsong Zhang

subject

AdultMalePathologymedicine.medical_specialtyCellOdontologíaBiologyMetastasisCell MovementChloride ChannelsBiomarkers TumorTumor Cells CulturedCarcinomamedicineHumansGene silencingGene SilencingGeneral DentistryAnoctamin-1Chloride channel activityMouth neoplasmOral Medicine and PathologyResearchCell migrationMiddle Aged:CIENCIAS MÉDICAS [UNESCO]medicine.diseaseCiencias de la saludNeoplasm Proteinsstomatognathic diseasesmedicine.anatomical_structureOtorhinolaryngologyUNESCO::CIENCIAS MÉDICASCarcinoma Squamous CellImmunohistochemistryFemaleMouth NeoplasmsSurgery

description

Objectives: The purpose of this study is to confirm that ANO1 correlates with occurrence and metastasis of OSCC. Study Design: Immunohistochemistry was used to detect the expression of ANO1 in 160 specimens of OSCC and normal tissues. Lentiviral silencing ANO1 was used in scc-25 cell line to study the cell migration and cell detachment. Results: Immunohistochemical staining revealed that ANO1 was expressed in a large majority (132 out of 160, 82.5%) of OSCC specimens and that the rate of ANO1 expression in OSCC was significantly higher than that of normal tissue ( P 0.05). Conclusions: Our study shows that abnormal expression of ANO1 correlated with the occurrence and metastasis of OSCC in clinical specimens and that silencing ANO1 greatly reduced migration ability of scc-25 cells. Calcium activated chloride channel activity of ANO1 promoted the cell migration. Thus, ANO1 could represent a new diagnostic biomarker and a potentially important therapeutic target of OSCC.

yearjournalcountryeditionlanguage
2013-01-21Medicina Oral Patología Oral y Cirugia Bucal
https://doi.org/10.4317/medoral.19076