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RESEARCH PRODUCT

Essential oil of Cyphostemma juttae (Vitaceae): Chemical composition and antitumor mechanism in triple negative breast cancer cells

Monica NotarbartoloManuela LabbozzettaPaola PomaMaurizio SajevaPietro Zito

subject

LeavesChemical CompositionTriple Negative Breast NeoplasmsPlant ScienceBiochemistryNF-κBAntioxidantsMass Spectrometrylaw.inventionAnalytical ChemistryTerpenechemistry.chemical_compound0302 clinical medicineSpectrum Analysis Techniquespro-oxidantlawBreast TumorsPlant defense against herbivoryMedicine and Health Sciencesantitumor0303 health sciencesMultidisciplinarybiologyTraditional medicineOrganic CompoundsPlant AnatomyQChromatographic TechniquesCell CycleRNF-kappa BLipidsChemistryOncologyVitaceaeCell Processes030220 oncology & carcinogenesisCyphostemmaPhysical SciencesMedicinecytotoxic effectterpenoidResearch ArticleCell SurvivalScienceVitaceaeResearch and Analysis Methodsessential oilGas Chromatography-Mass SpectrometryCell Growthphytol03 medical and health sciencesPhytolCyphostemma juttaeCell Line TumorBreast CancerOils VolatileHumansEssential oil030304 developmental biologyCell ProliferationCell growthTerpenesOrganic ChemistryChemical CompoundsBiology and Life SciencesCancers and NeoplasmsCell Biologybiology.organism_classificationAntineoplastic Agents PhytogenicPlant LeaveschemistrySettore BIO/03 - Botanica Ambientale E ApplicataSettore BIO/14 - FarmacologiaReactive Oxygen SpeciesOils

description

The genus Cyphostemma (Planch.) Alston (Vitaceae) includes about 150 species distrib- uted in eastern and southern Africa and Madagascar. Some species are used in traditional medicine and their biological activities, including antiproliferative effects against cancer cell lines, have been demonstrated. To date no investigations on Cyphostemma essential oils have been carried out. Essential oils, which play important roles in plant defenses have been demonstrated to be active in the treatment of several human diseases and to enhance bioavability of other drugs. The aim of this paper was to identify the chemical composition of the essential oil of the leaves of Cyphostemma juttae (Dinter & Gilg) Desc. and to verify some biological activities on two triple negative breast cancer cell lines (MDA-MB-231, SUM 149), characterized by the over-expression of the transcription factor NF-κB. In the essential oil, obtained by hydrodistillation and analysed by gas chromatography-mass spectrometry, 39 compounds were detected and with phytol (30%) dominating the chemical composition. C. juttae essential oil reduced cell growth and showed a pro-oxidant activity in both cell lines. Moreover, C. juttae essential oil caused a substantial decrease of NF-κB activation and consequently a significant reduction of some NF-κB target genes. The present study shows for the first time the cytotoxic properties of C. juttae essential oil and highlight its avail- ability to interfere with NF-κB pathway, suggesting a potential therapeutic use in triple nega- tive breast cancers (TNBCs) of this essential oil.

yearjournalcountryeditionlanguage
2019-03-28PLoS ONE
10.1371/journal.pone.0214594http://europepmc.org/articles/PMC6438575