6533b85afe1ef96bd12b9621
RESEARCH PRODUCT
Freezing and storage at -20 °C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis.
Hervé PellouxFeriel TouafekEmmanuelle Varlet-marieLaurence DelhaesFrédéric DalleSophie CassaingMarie-pierre Brenier-pinchartHélène YeraDenis FilisettiYvon SterkersYvon SterkersPatrick BastienPatrick Bastiensubject
Microbiology (medical)Time Factors[SDV]Life Sciences [q-bio]educationBiologyReal-Time Polymerase Chain ReactionSpecimen HandlingToxoplasma gondii DNAchemistry.chemical_compoundparasitic diseasesFreezingmedicineRetrospective Studiestoxoplasma gondiiDNA storageToxoplasma gondiiamniotic fluidGeneral MedicineDNA Protozoanmedicine.diseasebiology.organism_classificationVirologyToxoplasmosisDna storageMolecular analysisInfectious DiseasesReal-time polymerase chain reaction[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologychemistryMolecular Diagnostic Techniquescongenital toxoplasmosisNucleic acidMESH: DNA Protozoan/isolation&purification; Freezing; Molecular Diagnostic Technics/methods; Specimen Handling/methods; Toxoplasmosis/diagnosisreal-Time PCRToxoplasmaDNAToxoplasmosisdescription
Equipe EA MERS; International audience; Nucleic acid-based testing has become crucial for toxoplasmosis diagnosis. For retrospective (forensic or scientific) studies, optimal methods must be employed for DNA long-term storage. We compared Toxoplasma gondii detection before and after DNA storage using real-time PCR. No significant differences were found depending on duration or storage conditions at -20 °C or -80 °C.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-11-01 | Diagnostic microbiology and infectious disease |