6533b85afe1ef96bd12b964e
RESEARCH PRODUCT
DNA extraction from soils: old bias for new microbial diversity analysis methods.
Guy SoulasS. HalletJ. C. GermonRémi ChaussodLaurent PhilippotFabrice Martin-laurentG. Catrouxsubject
DNA BacterialRibosomal Intergenic Spacer analysisContext (language use)BiologyApplied Microbiology and BiotechnologyDNA RibosomalPolymerase Chain Reactionlaw.inventionSoillawRNA Ribosomal 16SBotanyMethodsRibosomal DNAPolymerase chain reactionSoil Microbiology[SDV.EE]Life Sciences [q-bio]/Ecology environmentErrataEcologyBacteriabusiness.industryRibosomal RNADNA extractionAmplified Ribosomal DNA Restriction AnalysisBiotechnology[SDV.EE] Life Sciences [q-bio]/Ecology environmentRNA Ribosomal 23SbusinessSoil microbiologyFood ScienceBiotechnologydescription
ABSTRACT The impact of three different soil DNA extraction methods on bacterial diversity was evaluated using PCR-based 16S ribosomal DNA analysis. DNA extracted directly from three soils showing contrasting physicochemical properties was subjected to amplified ribosomal DNA restriction analysis and ribosomal intergenic spacer analysis (RISA). The obtained RISA patterns revealed clearly that both the phylotype abundance and the composition of the indigenous bacterial community are dependent on the DNA recovery method used. In addition, this effect was also shown in the context of an experimental study aiming to estimate the impact on soil biodiversity of the application of farmyard manure or sewage sludge onto a monoculture of maize for 15 years.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-09-01 | Applied and environmental microbiology |