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RESEARCH PRODUCT
Removing striping artifacts in light-sheet fluorescence microscopy: a review
Francesco S. PavoneGiuseppe SancataldoCaroline MüllenbroichPietro RicciPietro RicciGiuseppe VitoGiuseppe De VitoLapo TurriniLapo TurriniVladislav GavryusevVladislav GavryusevLudovico SilvestriLudovico SilvestriLudovico Silvestrisubject
Materials scienceOptical sectioningBiophysicsBrain imaging01 natural sciences010309 optics03 medical and health sciencesOptics0103 physical sciencesFluorescence microscopeAnimalsMolecular Biology030304 developmental biology0303 health sciencesLight-sheet microscopyScatteringbusiness.industryRangingSample (graphics)FluorescenceMicroscopy FluorescenceLight sheet fluorescence microscopy3D microscopyStripingData striping3D microscopy; Brain imaging; Light-sheet microscopy; StripingArtifactsbusinessdescription
Abstract In recent years, light-sheet fluorescence microscopy (LSFM) has found a broad application for imaging of diverse biological samples, ranging from sub-cellular structures to whole animals, both in-vivo and ex-vivo, owing to its many advantages relative to point-scanning methods. By providing the selective illumination of sample single planes, LSFM achieves an intrinsic optical sectioning and direct 2D image acquisition, with low out-of-focus fluorescence background, sample photo-damage and photo-bleaching. On the other hand, such an illumination scheme is prone to light absorption or scattering effects, which lead to uneven illumination and striping artifacts in the images, oriented along the light sheet propagation direction. Several methods have been developed to address this issue, ranging from fully optical solutions to entirely digital post-processing approaches. In this work, we present them, outlining their advantages, performance and limitations.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2022-01-01 | Progress in Biophysics and Molecular Biology |