6533b85afe1ef96bd12b978f
RESEARCH PRODUCT
Simultaneous determination of olanzapine, clozapine and demethylated metabolites in serum by on-line column-switching high-performance liquid chromatography.
Sabine MaehrleinGerd DannhardtHarald WeigmannGodehard KrämerSebastian HärtterWerner KieferChristoph Hiemkesubject
Detection limitChromatographyChemistryMetaboliteReproducibility of ResultsGeneral ChemistryPirenzepineHigh-performance liquid chromatographySensitivity and SpecificityAcetic acidchemistry.chemical_compoundBenzodiazepinesAdsorptionPharmacokineticsOlanzapinemedicineHumansSpectrophotometry UltravioletQuantitative analysis (chemistry)ClozapineClozapineChromatography High Pressure Liquidmedicine.drugAntipsychotic Agentsdescription
An automated method for simultaneous routine quantification of the antipsychotic drugs clozapine, olanzapine and their demethylated metabolites is described. The method included adsorption on a cyanopropyl (CPS) coated clean-up column (10 microm; 10 x 2.0 mm I.D.), washing off interfering serum constituents to waste, and separation on C18 ODS Hypersil reversed phase material (5 microm; 250 x 4.6 mm I.D.) using acetonitrile-water-tetramethylethylenediamine (37:62.6:0.4, v/v/v) adjusted to pH 6.5 with concentrated acetic acid. UV-detection was performed at 254 nm. The limit of quantification was 10-20 ng/ml. Relative day to day standard variations ranged between 4.5 and 13.5%. The method is suitable for routine monitoring of olanzapine and clozapine including their demethylated metabolites.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-08-14 | Journal of chromatography. B, Biomedical sciences and applications |