6533b85afe1ef96bd12ba059
RESEARCH PRODUCT
Report of a european collaborative exercise comparing DNA typing results using a single locus VNTR probe
David J. WerrettBirthe EriksenA.d. KloostermanPeter GillS. WoodroffeCh. RittnerB. MevågWalter BärBernd BrinkmannVincenzo Lorenzo PascaliRolf FimmersJ. A. ThomsonH. SchmitterS. JonesPeter M. Schneidersubject
Restriction Mappingdata comparisonBiologyGenomePathology and Forensic Medicinechemistry.chemical_compoundVNTR probeRestriction mapforensic stain analysisHumansTypingAllelesGeneticsfragment size determinationNucleic Acid HybridizationReproducibility of ResultsDNAForensic MedicineSettore MED/43 - MEDICINA LEGALEEuropegenomic DNARestriction enzymechemistryGenetic markerDNA ProbesMolecular probeLawDNAdescription
A collaborative exercise was carried out in 1989 among 12 European forensic laboratories using the single locus VNTR probe pYNH24, the restriction enzyme HinfI, the same set of human genomic DNA samples, and a standardized DNA size marker. The objectives of the exercise were: (1) to study the degree of variation within and between laboratories, (2) to obtain information on requirements for technical standardization allowing the exchange of typing results and (3) to compare different approaches for the identification of allelic DNA fragments of unknown size. Each laboratory carried out up to 10 independent typing experiments using the same DNA samples. The results were analysed independently by two laboratories using three different methods. The results of the exercise demonstrate the correlation of typing that can be achieved within and between laboratories under conditions of minimal standardization.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-01-01 | Forensic Science International |