0000000000522801
AUTHOR
J. A. Thomson
Achievement of Interlaboratory Uniformity — A Summary of Work Carried out by the EDNAP Group
This paper describes a collaborative exercise intended to demonstrate whether uniformity of DNA profiles results could be achieved between different European laboratories. It was shown that this goal would be obtained provided that a common protocol was followed (specifically the use of a common electrophoretic buffer is the most important parameter).
A report of an international collaborative experiment to demonstrate the uniformity obtainable using DNA profiling techniques
This paper describes a collaborative exercise intended to demonstrate whether uniformity of DNA profile results could be achieved between different European laboratories. It was shown that this goal can be obtained provided that a common protocol is followed (specifically the use of a common electrophoretic buffer as being the most important parameter). Generally, lower molecular weight loci (with lower molecular weight fragments) such as YNH24 perform better than higher molecular weight loci such as MS43a. The results of the exercise are discussed in relation to the objectives of the European DNA profiling group (EDNAP).
Report of a european collaborative exercise comparing DNA typing results using a single locus VNTR probe
A collaborative exercise was carried out in 1989 among 12 European forensic laboratories using the single locus VNTR probe pYNH24, the restriction enzyme HinfI, the same set of human genomic DNA samples, and a standardized DNA size marker. The objectives of the exercise were: (1) to study the degree of variation within and between laboratories, (2) to obtain information on requirements for technical standardization allowing the exchange of typing results and (3) to compare different approaches for the identification of allelic DNA fragments of unknown size. Each laboratory carried out up to 10 independent typing experiments using the same DNA samples. The results were analysed independently…