6533b837fe1ef96bd12a1efb
RESEARCH PRODUCT
A report of an international collaborative experiment to demonstrate the uniformity obtainable using DNA profiling techniques
Vincenzo Lorenzo PascaliBirthe EriksenS. WoodroffeWalter BärBertrand LudesA.d. KloostermanB. MevågAngel CarracedoP. GillBernd BrinkmannM. RudlerJ. A. ThomsonPeter M. SchneiderH. SchmitterS. Jonessubject
GeneticsProtocol (science)Quality ControlElectrophoresis Agar GelDNA/bloodRestriction MappingComputational biologyDNABiologySettore MED/43 - MEDICINA LEGALEDNA FingerprintingPathology and Forensic MedicineDNA profilingMulticenter studyAutoradiographyHumansRestriction fragment length polymorphismLaboratoriesLawDNA Fingerprinting/standardsdescription
This paper describes a collaborative exercise intended to demonstrate whether uniformity of DNA profile results could be achieved between different European laboratories. It was shown that this goal can be obtained provided that a common protocol is followed (specifically the use of a common electrophoretic buffer as being the most important parameter). Generally, lower molecular weight loci (with lower molecular weight fragments) such as YNH24 perform better than higher molecular weight loci such as MS43a. The results of the exercise are discussed in relation to the objectives of the European DNA profiling group (EDNAP).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-02-01 |