6533b85bfe1ef96bd12bace2

RESEARCH PRODUCT

Proteomic insights into the immune response of the Colorado potato beetle larvae challenged with Bacillus thuringiensis.

Inmaculada García-roblesM. José López-galianoJessica De LomaPaz Boix-montesinosMiguel CapillaM. Dolores RealCarolina RausellMarcos VicentePablo CarriónIsabel Roger

subject

0106 biological sciences0301 basic medicinePore Forming Cytotoxic ProteinsProteomicsmedia_common.quotation_subjectImmunologyAntimicrobial peptidesBacillus thuringiensisInsect01 natural sciencesMicrobiology03 medical and health sciencesHemolysin ProteinsImmune systemBacillus thuringiensisHemolymphAnimalsGram-Positive Bacterial InfectionsSolanaceaemedia_commonLarvabiologyBacillus thuringiensis ToxinsMonophenol MonooxygenasefungiColorado potato beetleImmunitybiology.organism_classificationDietColeopteraEndotoxins010602 entomologyBiopesticideMicroRNAs030104 developmental biologyLarvaInsect ProteinsDevelopmental Biology

description

Bacillus thuringiensis (Bt) toxins constitute effective, environmentally safe biopesticides. Nevertheless, insects' tolerance to Bt is influenced by environmental factors affecting immunity. To understand larval immune response in the devastating coleopteran insect pest Colorado potato beetle (CPB), we undertook a proteomic analysis of hemolymph of non-treated control larvae and larvae consuming non-lethal doses of spore-crystal mixtures containing the coleopteran-active Cry3Aa toxin. Results revealed lower amount of proteins involved in insect growth and higher amount of immune response-related proteins in challenged insects, sustaining the larval weight loss observed. Additionally, we found a potential regulatory role of the evolutionary conserved miR-8 in the insect's immune response relying on antimicrobial peptides (AMPs) production. Upon toxin challenge, different patterns of hemolymph AMPs expression and phenoloxidase activity were observed in CPB larvae reared on different Solanaceae plants. This suggests that diet and diet-associated insect midgut microbiota might modulate this insects' tolerance to non-lethal doses of Bt.

10.1016/j.dci.2019.103525https://pubmed.ncbi.nlm.nih.gov/31655128