6533b85bfe1ef96bd12bb5bd
RESEARCH PRODUCT
Specific and highly efficient condensation of GC and IC DNA by polyaza pyridinophane derivatives
Marijana Radić StojkovićIvo PiantanidaLluis GuijarroSalvatore A. GazzeEnrique García-españaCristina Galiana-rosellóLewis FrancisFilip ŠUpljikaJorge González-garcíasubject
0301 basic medicineCircular dichroismStereochemistryPyridonesEnthalpySpermineCalorimetryMicroscopy Atomic ForceNucleic Acid DenaturationBiochemistryNucleobase03 medical and health scienceschemistry.chemical_compoundStructural BiologyPyridinophane compounds ; DNA/RNA binding ; GC-DNA condensation ; circular dichroism spectroscopyMolecular BiologyRNA Double-StrandedAnalytic ChemistryCircular DichroismOrganic ChemistryTemperatureRNAGeneral MedicineDNAChemistry030104 developmental biologyMonomerchemistryPolynucleotideNucleic Acid ConformationDNAdescription
Abstract Two bis-polyaza pyridinophane derivatives and their monomeric reference compounds revealed strong interactions with ds-DNA and RNA. The bis-derivatives show a specific condensation of GC- and IC-DNA, which is almost two orders of magnitude more efficient than the well-known condensation agent spermine. The type of condensed DNA was identified as ψ-DNA, characterized by the exceptionally strong CD signals. At variance to the almost silent AT(U) polynucleotides, these strong CD signals allow the determination of GC-condensates at nanomolar nucleobase concentrations. Detailed thermodynamic characterisation by ITC reveals significant differences between the DNA binding of the bis-derivative compounds (enthalpy driven) and that of spermine and of their monomeric counterparts (entropy driven). Atomic force microscopy confirmed GC-DNA compaction by the bis-derivatives and the formation of toroid- and rod-like structures responsible for the ψ-type pattern in the CD spectra.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-01-01 |