6533b85bfe1ef96bd12bbf73
RESEARCH PRODUCT
Isolation of endothelin A receptor from bovine lungs.
Simone HickJasminka Godovac-zimmermannsubject
PharmacologyGlycosylationReceptors EndothelinBiologyIn vitroRatsMolecular Weightchemistry.chemical_compoundBiochemistryAffinity chromatographychemistrybiology.proteinAnimalsCattleRabbitsAntibodyBradykinin receptorCardiology and Cardiovascular MedicineEndothelin receptorReceptorLungIntracellulardescription
We isolated endothelin receptor A (ET A ) from bovine lungs in a single-step purification procedure using antibodies raised against synthetic peptides that correspond to extra- and intracellular domains of the rat bradykinin receptor. Two receptor species of 55 and 35 kDa were isolated and subjected to N-terminal microsequencing. The difference between the observed and expected molecular weight species suggests that bovine ET A receptor is glycosylated.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-01-01 | Journal of cardiovascular pharmacology |