6533b85cfe1ef96bd12bbff7

RESEARCH PRODUCT

EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness

Angela L. Riffo-camposMarisol HuertaDesamparados RodaValentina GambardellaGuillermo AyalaNoelia TarazonaAndrés CervantesSusana RosellóCarolina Martínez-ciarpagliniGerardo López-rodasFernanda Mariel RodríguezLuis FrancoJosefa CastilloF. Gimeno-valienteJ. Montón-bueno

subject

AdultMale0301 basic medicineEpithelial-Mesenchymal TransitionColorectal cancerIn silicolcsh:MedicineNerve Tissue ProteinsBiologyArticle//purl.org/becyt/ford/3.3 [https]03 medical and health sciences0302 clinical medicinemedicinecancerHumansNeoplasm InvasivenesshumanProspective StudiesEpithelial–mesenchymal transitionlcsh:ScienceAgedCell ProliferationNeoplasm StagingcolorectalAged 80 and overRegulation of gene expressionMultidisciplinaryCell growthlcsh:RMethylationMiddle Agedmedicine.diseaseColorectal cancerNeoplasm ProteinsUp-RegulationEPDR1Gene Expression Regulation Neoplastic030104 developmental biologyCpG siteCell culture030220 oncology & carcinogenesisCancer research//purl.org/becyt/ford/3 [https]Femalelcsh:QColorectal NeoplasmsTranscription

description

The finding of novel molecular markers for prediction or prognosis of invasiveness in colorectal cancer (CRC) constitutes an appealing challenge. Here we show the up-regulation of EPDR1 in a prospective cohort of 101 CRC patients, in a cDNA array of 43 patients and in in silico analyses. EPDR1 encodes a protein related to ependymins, a family of glycoproteins involved in intercellular contacts. A thorough statistical model allowed us to conclude that the gene is significantly up-regulated in tumour tissues when compared with normal mucosa. These results agree with those obtained by the analysis of three publicly available databases. EPDR1 up-regulation correlates with the TNM staging parameters, especially T and M. Studies with CRC cell lines revealed that the methylation of a CpG island controls EPDR1 expression. siRNA knocking-down and overexpression of the gene following transient plasmid transfection, showed that EPDR1 favours cell proliferation, migration, invasiveness and adhesion to type I collagen fibres, suggesting a role in epithelial to mesenchymal transition. Both statistical and functional analysis correlated EPDR1 overexpression with invasiveness and dissemination of tumour cells, supporting the inclusion of EPDR1 in panels of genes used to improve molecular subtyping of CRC. Eventually, EPDR1 may be an actionable target. Fil: Gimeno Valiente, F.. No especifíca; Fil: Riffo Campos, Á. L.. Universidad de La Frontera; Chile Fil: Ayala, G.. Universidad de Valencia; España Fil: Tarazona, N.. Universidad de Valencia; España Fil: Gambardella, V.. Universidad de Valencia; España Fil: Rodríguez, Fernanda Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Huerta, M.. Universidad de Valencia; España Fil: Martínez-Ciarpaglini, C.. Universidad de Valencia; España Fil: Montón Bueno, J.. Universidad de Valencia; España Fil: Roselló, S.. Universidad de Valencia; España Fil: Roda, D.. Universidad de Valencia; España Fil: Cervantes, A.. Universidad de Valencia; España Fil: Franco, L.. Universidad de Valencia; España Fil: López Rodas, G.. Universidad de Valencia; España Fil: Castillo, J.. Universidad de Valencia; España

yearjournalcountryeditionlanguage
2020-02-01Scientific Reports
https://doi.org/10.1038/s41598-020-60476-7