6533b85cfe1ef96bd12bcaab
RESEARCH PRODUCT
Evaluation of mechanical and morphologic features of PLLA membranes as supports for perfusion cells culture systems
Valerio BrucatoSalvatore MontesantoV. La Carrubbasubject
Materials scienceScanning electron microscopePolyestersCell Culture TechniquesPolyesterBioengineeringNanotechnology02 engineering and technologyCondensed Matter Physic010402 general chemistry01 natural sciencesDip-coatingCell LineBiomaterialsElastic ModulusTensile StrengthDynamic modulusHumansMechanics of MaterialPorosityElastic modulusMechanical PhenomenaElastic ModuluEpithelial CellMechanical Engineeringtechnology industry and agricultureTemperatureCell adhesionEpithelial CellsMembranes ArtificialDynamic mechanical analysis021001 nanoscience & nanotechnology0104 chemical sciencesPLLA membranePolyesterPerfusionMembraneChemical engineeringMechanics of MaterialsMaterials Science (all)Stress Mechanical0210 nano-technologyMechanical propertieCell Culture TechniquePorosityHumandescription
Abstract Porous biodegradable PLLA membranes, which can be used as supports for perfusion cell culture systems were designed, developed and characterized. PLLA membranes were prepared via diffusion induced phase separation (DIPS). A glass slab was coated with a binary PLLA–dioxane solution (8 wt.% PLLA) via dip coating, then pool immersed in two subsequent coagulation baths, and finally dried in a humidity-controlled environment. Surface and mechanical properties were evaluated by measuring pore size, porosity via scanning electron microscopy, storage modulus, loss modulus and loss angle by using a dynamic mechanical analysis (DMA). Cell adhesion assays on different membrane surfaces were also performed by using a standard count method. Results provide new insights into the foaming methods for producing polymeric membranes and supply indications on how to optimise the fabrication parameters to design membranes for tissue cultures and regeneration.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-11-04 |