6533b85dfe1ef96bd12be75a
RESEARCH PRODUCT
Characterization of polymorphisms in the toxin A and B genes of Clostridium difficile.
Florian SoehnRita Laufenberg-feldmannVeit BraunMiha JancChristoph Von Eichel-streiberMonika HofstetterMaja RupnikMaja Rupniksubject
GeneticsPolymorphism GeneticClostridioides difficileBacterial ToxinsClostridium difficile toxin AClostridium difficile toxin BBiologyClostridium difficileMicrobiologyMolecular biologyPolymerase Chain Reactionlaw.inventionRestriction siteEnterotoxinsBacterial ProteinslawGenes BacterialGenotypeGeneticsTypingRestriction fragment length polymorphismMolecular BiologyPolymerase chain reactionPolymorphism Restriction Fragment Lengthdescription
We have used six independent polymerase chain reactions (A1–A3 and B1–B3) for amplification of the entire sequence of the two toxin genes tcdA and tcdB of several Clostridium difficile strains. With this approach we have detected (1) restriction site polymorphisms which are distributed all over the genes, and (2) deletions that could be found only in tcdA. Characteristic differences between strains were mainly focused to the 5′ third of tcdB (B1 fragment) and/or the 3′ third of tcdA (A3 fragment). The possible use of our approach for typing of C. difficile toxin genes is discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-01-17 | FEMS microbiology letters |