6533b85dfe1ef96bd12be878
RESEARCH PRODUCT
Real-time polarimetric biosensing using macroporous alumina membranes
Geoffrey W. PlattLaura SolaMarcella ChiariMarcus J. SwannJuan P. Martínez-pastorJesús ÁLvarezMarina CretichDaniel Hillsubject
AnalyteMaterials scienceNacl solutionsAlumina membranesAnalytical chemistryfood and beveragesSmall sampleRabbit (nuclear engineering)02 engineering and technology021001 nanoscience & nanotechnology01 natural sciences010309 opticsMembrane0103 physical sciences0210 nano-technologyBiosensorRefractive indexdescription
We report the first demonstration of real-time biosensing in free standing macroporous alumina membranes. The membranes with their 200 nm diameter pores are ideal candidates for biosensing applications where fast response times for small sample volumes are needed as they allow analytes to flow through the pores close to the bioreceptors immobilized on the pores walls. A bulk refractive index sensitivity of 5.2x10 -6 refractive index units was obtained from signal responses to different concentrations of NaCl solutions flowing through the pores. Finally, after functionalizing the alumina pore surfaces with an epoxysilane and then spotting it with β-Lactoglobulin protein, the interactions between the β-lactoglobulin and rabbit anti-β-lactoglobulin, as well as the interaction between the rabbit anti-β-lactoglobulin and a secondary antibody anti-rabbit Immunoglobulin G were monitored in real-time.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-05-28 | SPIE Proceedings |