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RESEARCH PRODUCT

The affinity for oxygen of polyphenoloxidase in grapes

Ferdinand RadlerE. Torokfalvy

subject

WineChromatographyChemistrySubstrate (chemistry)chemistry.chemical_elementGeneral ChemistryFood scienceBiochemistryOxygenIndustrial and Manufacturing EngineeringFood ScienceBiotechnology

description

The influence of oxygen on the reaction of polyphenoloxidase in grapes was determined. Using crude extracts of grapes with a sufficiently high polyphenoloxidase activity on a substrate of pyrocatechol, apparentKm values for oxygen, at 25° C were calculated as 1.1 × 10−4mol (or 9% 02) for Sultana, and 1 × 10−4mol (or 8% O2) for Doradillo grapes. The importance of these figures for wine making is discussed.

https://doi.org/10.1007/bf01810560