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RESEARCH PRODUCT
Characterization of Different Deoxyribonucleases in Human Lymphocytes
Rudolf K. ZahnStörger HHans-joachim BreterZöllner Ejsubject
DNA BacterialCytoplasmUltraviolet RaysPolyacrylamideNucleic Acid DenaturationGeneral Biochemistry Genetics and Molecular Biologychemistry.chemical_compoundHydrolysismedicineHumansLymphocytesCell NucleusDeoxyribonucleasesSubstrate (chemistry)DeoxyribonucleaseDNAHydrogen-Ion ConcentrationElectrophoresis DiscRadiation Effectsmedicine.anatomical_structurechemistryBiochemistryCytoplasmDeoxyribonucleasesNucleusDNAdescription
Abstract Deoxyribonucleases, Disc Electrophoresis, Lymphocytes Four groups of deoxyribonuclease activities from human lymphocytes have been characterized by deoxyribonuclease assay in DNA-containing polyacrylamide gels following their separation by disc-electrophoresis. All activities hydrolyse DNA endonucleolytically. One neutral deoxyribo nuclease found in the cytoplasmic fraction prefers native or UV-irradiated DNA over denatured DNA as substrate and is a 5′-monoester former. Two groups of acid deoxyribonuclease activities are detectable in the nuclear fraction. Both are 3′-monoester formers. One is as well active with denatured DNA as with native DNA, the other one shows the same activity with native and UV-irradiated DNA but lower activity with denatured DNA. An alkaline deoxyribonuclease activity, also localized in the nucleus, is a 5′ -monoester DNA as substrate.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1975-12-01 | Zeitschrift für Naturforschung C |