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RESEARCH PRODUCT

Optimization of Cell Growth on Bacterial Cellulose by Adsorption of Collagen and Poly-L-Lysine

Clara M. GómezFernando G. TorresMario CulebrasOmar P. TroncosoM. Carmen BañóCristian J. Grande

subject

Materials sciencePolymers and PlasticsCell growthGeneral Chemical EngineeringChinese hamster ovary cellSubstrate (chemistry)complex mixturesAnalytical Chemistrychemistry.chemical_compoundTissue cultureAdsorptionchemistryBiochemistryBacterial celluloseBiophysicsbacteriaViability assayCell adhesion

description

Poly-L-lysine and collagen were separately added to bacterial cellulose (BC) nanofibers. The ionic surface charge had been previously modified in order to promote the adsorption of poly-L-lysine and collagen. Cell adhesion of Chinese hamster ovary (CHO) cells on BC surfaces was confirmed by removing unattached cells from the BC substrates. Cell viability was calculated and it was determined that both poly-L-lysine-BC and collagen-BC substrates are viable for cell growth. The results showed that the cell viability in poly-L-lysine modified BC substrate is similar to the one observed in polystyrene tissue culture plates.

yearjournalcountryeditionlanguage
2015-01-06International Journal of Polymeric Materials and Polymeric Biomaterials
https://doi.org/10.1080/00914037.2014.958829