Reduced apoptosis of CD8+ T-Lymphocytes in the airways of smokers with mild/moderate COPD

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RESEARCH PRODUCT

Reduced apoptosis of CD8+ T-Lymphocytes in the airways of smokers with mild/moderate COPD

Alan L. James Alan L. James Elisabetta Pace Marina Saetta Liboria Siena John G. Elliot Maria R. Bonsignore Simonetta Baraldo Mark Gjomarkaj Andreina Bruno

subject

Pulmonary and Respiratory Medicine Male apoptosis cytotoxic T-lymphocytes inflammation lung tissue Inflammation Apoptosis Settore MED/10 - Malattie Dell'Apparato Respiratorio CD8-Positive T-Lymphocytes FEV1/FVC ratio Pulmonary Disease Chronic Obstructive Submucosa Forced Expiratory Volume Parenchyma medicine Humans Lung Lung tissue Inflammation COPD Analysis of Variance Lung business.industry Cytotoxic T-lymphocytes Smoking Middle Aged respiratory system medicine.disease Immunohistochemistry respiratory tract diseases medicine.anatomical_structure Immunology behavior and behavior mechanisms Female medicine.symptom Airway business CD8

description

SummaryChronic obstructive pulmonary disease (COPD) is characterised by chronic inflammation in airways and lung parenchyma. CD8+ T-lymphocytes, crucial effector and regulatory cells in inflammation, are increased in the central and peripheral airways in COPD. The aim of this study was to assess the role of apoptosis in the accumulation of CD8+ T-lymphocytes within the airway wall in COPD. We examined the submucosa of transverse sections of central and peripheral airways from post-operative tissues from non-smokers (n = 16), smokers with normal lung function (n = 16), smokers with mild/moderate COPD (n = 16), and smokers with severe/very severe COPD (n = 9). TUNEL and immunohistochemistry techniques were used to identify apoptosis and cell phenotype, respectively. The percentage of apoptotic CD8+ T-lymphocytes was significantly lower (p < 0.0001) in smokers with mild/moderate COPD than in non-smokers, smokers with normal lung function, and smokers with severe/very severe COPD, and was positively related to values of FEV1 and FEV1/FVC ratio, both in central and in peripheral airways. These data suggest that reduced apoptosis of CD8+ T-lymphocytes may be an important mechanism that contributes to the accumulation of these cells in the airway submucosa in smokers with mild/moderate COPD.

year	journal	country	edition	language
2011-10-01	Respiratory Medicine

10.1016/j.rmed.2011.04.014 http://dx.doi.org/10.1016/j.rmed.2011.04.014