6533b85ffe1ef96bd12c2791
RESEARCH PRODUCT
Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples.
Eulogio ValentínMaría ÁNgeles Tormo-másFélix SancenónLuis PlaElena AznarSara Santiago-felipeJavier PemánRamón Martínez-máñezAlba Ruiz-gaitánsubject
oligonucleotide0301 basic medicineEpidemiology030106 microbiologyImmunologyRapid diagnosismacromolecular substancesbiosensorCandida auris Nanoporous anodic alumina biosensor molecular gates oligonucleotide rapid diagnosisMicrobiologyMicrobiology03 medical and health sciencesQUIMICA ORGANICAVirologyOligonucleotideDrug DiscoveryQUIMICA ANALITICArapid diagnosisNosocomial outbreakChemistryNanoporousOligonucleotideQUIMICA INORGANICAGeneral MedicineCandida aurisMolecular gates030104 developmental biologyInfectious DiseasesCandida aurisParasitologyNanoporous anodic aluminaBiosensormolecular gatesBiosensordescription
[EN] Candida auris has arisen as an important multidrug-resistant fungus because of several nosocomial outbreaks and elevated rates of mortality. Accurate and rapid diagnosis of C. auris is highly desired; nevertheless, current methods often present severe limitations and produce misidentification. Herein a sensitive, selective, and time-competitive biosensor based on oligonucleotide-gated nanomaterials for effective detection of C. auris is presented. In the proposed design, a nanoporous anodic alumina scaffold is filled with the fluorescent indicator rhodamine B and the pores blocked with different oligonucleotides capable of specifically recognize C. auris genomic DNA. Gate opening modulation and cargo delivery is controlled by successful DNA recognition. C. auris is detected at a concentration as low as 6 CFU/mL allowing obtaining a diagnostic result in clinical samples in one hour with no prior DNA extraction or amplification steps.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-01-01 |