6533b860fe1ef96bd12c3010

RESEARCH PRODUCT

Aptamer-embedded DNA origami cage for detecting (glycated) hemoglobin with a surface plasmon resonance sensor

Brent D. CameronHeini IjäsHeini IjäsDong-shik KimBoxuan ShenVeikko LinkoSurachet DuanghathaipornsukMauri A. Kostiainen

subject

Materials scienceSurface plasmon resonance sensorAptamerAptamerDissociation constant02 engineering and technologybiosensorit010402 general chemistry01 natural sciencesSurface plasmon resonance sensorchemistry.chemical_compoundGlycated hemoglobinnanorakenteethemoglobiiniDNA origamiGeneral Materials ScienceSurface plasmon resonanceMechanical EngineeringDNA021001 nanoscience & nanotechnologyCondensed Matter Physics0104 chemical sciencesplasmonitDissociation constantchemistryMechanics of MaterialsBiophysicsDNA origamiHemoglobinGlycated hemoglobin0210 nano-technologyDNA

description

DNA origami-based cages functionalized with aptamer motifs, were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb. peerReviewed

yearjournalcountryeditionlanguage
2020-09-15Materials Letters
https://doi.org/10.1016/j.matlet.2020.128141