6533b860fe1ef96bd12c385c

RESEARCH PRODUCT

Die bestimmung von sedimentationskoeffizient, diffusions-koeffizient und molekulargewicht von α-amylasen nach der methode des aktivitätstransportes

G.v SchulzH. MutzbauerD. Kranz

subject

Activity measurementsChromatographyMolecular massPartial specific volumeChemistryDiffusionPolymer chemistryBiochemistry Genetics and Molecular Biology (miscellaneous)

description

Abstract Sedimentation and diffusion coefficients determined by optical methods on pure (crystallized) enzymes are compared with those obtained by activity measurements on the unpurified enzymes. On performing the experiments under suitable conditions, results of the two methods are in good agreement. The calculation of the molecular weights of the unpurified enzymes is somewhat uncertain because the partial specific volume in most cases is not accurately known.

https://doi.org/10.1016/0926-6585(65)90141-x