6533b861fe1ef96bd12c44d6

RESEARCH PRODUCT

Comparison of binding properties and early biological effects of elicitins in tobacco cells

Stéphane BourqueMarie-noëlle BinetAngela Lebrun-garciaPierre RicciMichel PonchetAlain Pugin

subject

0106 biological sciencesPhysiologyNicotiana tabacumPlant Science01 natural sciences[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics03 medical and health sciencesCell surface receptor[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants geneticsGeneticsExtracellularBinding siteComputingMilieux_MISCELLANEOUS030304 developmental biology0303 health sciencesbiologyBinding proteinElicitinTECHNIQUE DES TRACEURSbiology.organism_classificationElicitorBiochemistryCULTURE DE CELLULESignal transduction010606 plant biology & botanyResearch Article

description

Abstract Elicitins are a family of small proteins secreted by Phytophthora species that have a high degree of homology and elicit defense reactions in tobacco (Nicotiana tabacum). They display acidic or basic characteristics, the acidic elicitins being less efficient in inducing plant necrosis. In this study we compared the binding properties of four elicitins (two basic and two acidic) and early-induced signal transduction events (Ca2+ influx, extracellular medium alkalinization, and active oxygen species production). The affinity for tobacco plasma membrane-binding sites and the number of binding sites were similar for all four elicitins. Furthermore, elicitins compete with one another for binding sites, suggesting that they interact with the same receptor. The four elicitins induced Ca2+ influx, extracellular medium alkalinization, and the production of active oxygen species in tobacco cell suspensions, but the intensity and kinetics of these effects were different from one elicitin to another. As a general observation the concentrations that induce similar levels of biological activities were lower for basic elicitins (with the exception of cinnamomin-induced Ca2+ uptake). The qualitative similarity of early events induced by elicitins indicates a common transduction scheme, whereas fine signal transduction tuning is different in each elicitin.

https://hal.inrae.fr/hal-02688408