6533b861fe1ef96bd12c4e89
RESEARCH PRODUCT
Ubiquitin-like epitopes associated with Candida albicans cell surface receptors
Daniel GozalboAna M. CerveraW L ChaffinJosé P. MartínezPilar SepúlvedaJose L. Lopez-ribotsubject
GlycosylationImmunologyReceptors Cell SurfaceMicrobiologyEpitopeEpitopesUbiquitinCell surface receptorCandida albicansAnimalsCandida albicansReceptorUbiquitinsAntiserumbiologyImmune Serabiology.organism_classificationMolecular biologyCorpus albicansInfectious DiseasesBiochemistryPolyclonal antibodiesbiology.proteinParasitologyRabbitsResearch Articledescription
We have recently reported the cloning of a Candida albicans polyubiquitin gene and the presence of ubiquitin in the cell wall of this fungus. The polyubiquitin cDNA clone was isolated because of its reactivity with antibodies generated against the candidal 37-kDa laminin-binding protein. In the present study, we have further investigated the relationship between ubiquitin and cell wall components displaying receptor-like activities, including the 37-kDa laminin receptor, the 58-kDa fibrinogen-binding mannoprotein, and the candidal C3d receptor. Two-dimensional electrophoretic analysis and immunoblot experiments with antibodies against ubiquitin and the individually purified receptor-like molecules confirmed that these cell surface components are ubiquitinated. In an enzyme-linked immunosorbent assay, polyclonal antisera to each receptor reacted with ubiquitin, thus demonstrating that the purified receptor preparations used as immunogens contained ubiquitin-like epitopes. It is proposed that ubiquitin may play a role in modulating the activity of these receptors and in the interaction of C. albicans cells with host structures.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-10-01 |