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RESEARCH PRODUCT
Guanosine-Mediated Anxiolytic-Like Effect: Interplay with Adenosine A1 and A2A Receptors
Renata CiccarelliGiuseppa MudòPatrizia Di IorioRoberta GarozzoFulvio PlesciaDaniele Filippo CondorelliDaniele F. CondorelliMonica FrinchiFrancisco CiruelaNatale BelluardoValentina Di LibertoFrancesco CaciagliVincenzo VerdiVincenzo VerdiMaria Grillosubject
LightPharmacologyAnxietySettore BIO/09 - FisiologiaHippocampuslcsh:Chemistrychemistry.chemical_compound0302 clinical medicineReceptorlcsh:QH301-705.5Spectroscopycaffeine0303 health sciencesBehavior AnimalRGeneral MedicineDarkness3. Good healthComputer Science ApplicationsadenosineCCPA[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]A<sub>1</sub>RCaffeineA1Rmedicine.drugReceptor Adenosine A2A1GuanosineCatalysisArticleInorganic Chemistry03 medical and health sciencesAmedicineAnimals[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]Physical and Theoretical ChemistryBinding site2AMolecular Biology030304 developmental biologyDose-Response Relationship DrugReceptor Adenosine A1behaviorOrganic ChemistryCell MembraneAntagonistAdenosineAdenosine receptorRatsguanosineA<sub>2A</sub>Rlcsh:Biology (General)lcsh:QD1-999chemistryA2AR030217 neurology & neurosurgerydescription
Acute or chronic administration of guanosine (GUO) induces anxiolytic-like effects, for which the adenosine (ADO) system involvement has been postulated yet without a direct experimental evidence. Thus, we aimed to investigate whether adenosine receptors (ARs) are involved in the GUO-mediated anxiolytic-like effect, evaluated by three anxiety-related paradigms in rats. First, we confirmed that acute treatment with GUO exerts an anxiolytic-like effect. Subsequently, we investigated the effects of pretreatment with ADO or A1R (CPA, CCPA) or A2AR (CGS21680) agonists 10 min prior to GUO on a GUO-induced anxiolytic-like effect. All the combined treatments blocked the GUO anxiolytic-like effect, whereas when administered alone, each compound was ineffective as compared to the control group. Interestingly, the pretreatment with nonselective antagonist caffeine or selective A1R (DPCPX) or A2AR (ZM241385) antagonists did not modify the GUO-induced anxiolytic-like effect. Finally, binding assay performed in hippocampal membranes showed that [3H]GUO binding became saturable at 100&ndash
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-12-05 |