6533b861fe1ef96bd12c4f07

RESEARCH PRODUCT

Increase by NO synthase inhibitors of acetylcholine release from guinea-pig myenteric plexus

Heinz KilbingerD. Wolf

subject

medicine.medical_specialtyGuinea PigsMyenteric PlexusArginineNitric OxideNitroarginineNitric oxideGuinea pigchemistry.chemical_compoundInternal medicinemedicineAnimalsCholineEvoked PotentialsMyenteric plexusPharmacologyomega-N-MethylargininebiologyMuscle SmoothGeneral MedicineAcetylcholineElectric StimulationNitric oxide synthaseEndocrinologychemistryEnzyme inhibitorbiology.proteinLiberationAmino Acid OxidoreductasesNitric Oxide SynthaseAcetylcholinemedicine.drug

description

The effects of nitric oxide (NO) synthase inhibitors on the electrically evoked release of [3H]acetylcholine were studied in guinea-pig myenteric plexus preparations preincubated with [3H]choline. NG-monomethyl-L-arginine (EC50 5.3 mumol l-1) and NG-nitro-L-arginine (EC50 1.3 mumol l-1) concentration-dependently increased the evoked release of [3H]acetylcholine without affecting the basal outflow. The facilitatory effect of NG-mono-methyl-L-arginine was prevented by L-arginine but not by D-arginine. The results suggest that endogenous NO inhibits the depolarisation-evoked release of acetylcholine.

yearjournalcountryeditionlanguage
1994-05-01Naunyn-Schmiedeberg's Archives of Pharmacology
https://doi.org/10.1007/bf00169145